摘要
旨在研究微囊藻水华对鱼类的生态毒性及其机理.采用饲喂微囊藻水华浮沫的暴露方法对泥鳅进行口服暴露28 d,剂量为经腹腔注射微囊藻细胞抽取物LD50的1/10[即75 mg细胞抽提物/(kg.体重.d),约为10μgMC-RR/(kg.体重.d)].生长测定结果表明,饲喂微囊藻明显抑制泥鳅的生长,但没有改变实验鱼的肝体比.透射电镜观察发现,实验鱼肝细胞结构有明显的改变,细胞器受到损伤.所观察到的超微结构变化主要是内质网膨胀、细胞核变形等,但线粒体结构没有明显变化.显微观察还发现了脾细胞结构的变化,说明微囊藻毒素对泥鳅脾脏也有损伤作用.蛋白磷酸酶抑制测定法检测发现,受试泥鳅肌肉中没有微囊藻毒素的积累,可能是由于低剂量的微囊藻毒素暴露没有引起生物富集作用.
The subchronic oral toxicity of microcystins in loach has been studied in the present paper. The fish with mean body weight of （8.65±1.96） g were orally exposed to Microcystis by feeding with bloom scum at a day-dose of 10 μg microcystin-RR / kg body weight under laboratory conditions for 28 days. Growth assay results show that microcystins inhibit the growth of loach, but fail to change the fish hepatosomatic index. Ultrastructural examination by electron microscopy not only reveal damages in hepatocytes derived from the treated fish, but also in splenic cells. No bioaccurnulation of microcystin in the muscle of loach is found by the method of protein phosphatase inhibition assay after 28 days of microcystin-exposure.
出处
《河南师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第1期92-95,148,共5页
Journal of Henan Normal University(Natural Science Edition)
基金
韩国科学工程基金会(KOSEF)博士后项目(AP-01)
韩国科学工程基金会项目(R04-2003-000-10130-0)
河南省高校杰出科研人才创新工程项目(2006KYCX021)
教育部留学回国人员科研启动基金[2006]331
河南省动物学重点学科资助
关键词
微囊藻
泥鳅
口服亚慢性毒性
Microcystis
Misgurnus mizolepis
subchronic oral toxicity
