芦笋雌雄性别间同工酶酶谱的比较分析

Comparatively Analysis on Isozyme Patterns of Asparagus offcinalis L. Male and Female Plants

采用聚丙烯酰胺不连续垂直平板凝胶电泳(PAGE)技术,对芦笋雌雄株叶片中的过氧化物酶(POD)、酯酶(EST)、谷氨酸脱氢酶(GDH)和淀粉酶(AMY)同工酶进行了比较分析.结果表明,芦笋雌株与雄株之间的过氧化物酶及酯酶同工酶酶谱存在显著差异,过氧化物酶在雄株中比在雌株中多了Rf值为0.86的一条酶带;酯酶在雄株中多了Rf值为0.18和0.30的两条酶带.谷氨酸脱氢酶和淀粉酶在雌雄株间具有相同的酶谱带,没有表现出性别差异.

In this paper, Peroxidase （POD）, Esterase （EST）, Glutamate Dehydrogenase （GDH） and Amylase （AMY） isozyme of Asparagus offcinalis L. were analysed by polyacrylamide gel electrophoresis （PAGE）. The results indicate that the patterns of GDH and AMY isozyme are identical between male and female strains. While the patterns of POD and EST isozyme are obviously different in male and female-plant, that is, male-asparagus has one band （Rf=0.86） more of POD, and two bands （Rf=0.18, Rf=0.30） more of EST, which do not exist at all in female-plant. So they can be the markers in sex i- dentification of asparagus of fcinalis L. .