锌和锰拮抗二氧化硅细胞毒性作用的研究

SDUDY ON ANTAGONISM OF ZINC AND MANGANESE ON THE CYTOTOXICITY OF SILICA

[目的]研究葡萄糖酸锌和氯化锰单独及联合拮抗二氧化硅对肺泡巨噬细胞的毒性作用。[方法]采用肺灌洗法获得肺泡巨噬细胞(1×106/ml),与二氧化硅同时加入不同浓度的葡萄糖酸锌、氯化锰以及葡萄糖酸锌+氯化锰溶液,共同培养18h后检测巨噬细胞中谷胱甘肽过氧化物酶、过氧化氢酶的活性和过氧化氢的含量。[结果]葡萄糖酸锌和氯化锰,以及两者合用都具有拮抗二氧化硅细胞毒性的作用,两者最佳组合是1.5μg/ml葡萄糖酸锌+0.25μg/ml氯化锰。[结论]本实验证实葡萄糖酸锌、氯化锰在体外可有效拮抗二氧化硅粉尘的细胞毒性作用,降低肺泡巨噬细胞的过氧化氢的含量以及升高谷胱甘肽过氧化物酶和过氧化氢酶的活性,两者共同作用更为明显,并找到了最佳剂量组合。这一研究结果,对进一步探索矽肺防治新途径具有一定的理论意义和实际应用价值。

[Objective]To investigate the toxic effects of Zinc gluconate and Manganous chloride which were used solely and together to oppose the toxicity of silica on alveolar macrophage. [Methods] Alveolar macrophages were cultured with silica, at the same time Zinc gluconate, Manganous chloride and Zinc gluconate together with Manganous chloride were added re spectively. Glutathine Peroxidase（GSH-Px）, H2O2 and Catalase （CAT） were measured. [Results] Zinc gluconate, Manganous chloride and Zinc gluconate together with Manganous chloride had the effects to oppose the toxicity of silica on alveolar macrophage. The most suitable combination of the two substances should be middle dosage of Zinc gluconate （ 1.5μg/ml） with middle dosage of Manganous chloride （0.25μg/ml）. [Conclusion] Zinc gluconate and Manganous chloride which are used solely and together can reduce the level of H2O2, and increase GSH-Px and CAT on alveolar macrophage exposed to silica.