有机硒增强巨噬细胞处理BCG能力的初步探讨

INITIAL DISCUSSION ABOUT ORGANIC SELENIUM STRENGTHENING THE DISPOSAL ABILITY OF MACROPHAGE ON BCG

[目的]从体外细胞水平探讨有机硒对受BCG感染后的巨噬细胞产生NO的能力、生存能力和处理细菌能力的影响。[方法]使用Griess法、MTT法、Ziehl-heelson染色法及活菌计数法,通过检测各实验组小鼠腹腔巨噬细胞和巨噬细胞系Raw264.7细胞在不同硒浓度的培养液内感染BCG后的NO释放水平、细胞的存活率和载菌量来分析硒对巨噬细胞抗结核菌天然防御作用的影响。[结果]含硒感染孔的NO产量显著高于无硒感染孔,BCG+Se组以25～100ng/ml含硒孔NO增高显著(P﹤0.05);含硒感染孔的Mφ成活率均高于无硒感染孔(P﹤0.05);含硒孔荷菌量均低于无硒孔,但吞菌4h的活菌计数的结果却恰恰相反。[结论]有机硒对Mφ的抗结核天然防御免疫功能有促进作用,且有剂量依赖关系。同时,硒对巨噬细胞有应激保护作用。

[Objective] To discuss about Se influencing the abilities of macrophages infected by BCG in the fields of NO-producing, sun＇ival and treatment of the bacterium in vitro. [Methods] The peritoneal macrophages collected from all groups and Raw264.7 cells were cuhivated in various concentrations of selenium. The levels of nitrogen monoxidum （NO） released by Mφ after infection with BCG, which was measured by Griess method. The survival rate was determined by MTT assay and the bacteria burden in Mφ was determined by Ziehl-heelson staining method and colony counting method. [ Results] The levels of NO released by infected Mφ were higher than those without Se holes, specially those released by infected Mφ in the BCG＋Se group with 25-100ng/ml Se were significantly different from those without Se holes （P〈0.05）. Meanwhile, survival rates of infected macrophages in RPMI-1640 containing different concentrations of Se were higher than those without Se holes; the bacteria amount in Mφ with Se holes were lower than that without Se holes. However, the results of viable bacteria count in macrophages after swallowing BCG 4 hours were paradoxical. [Conclusion] The results suggest that selenium could promote the maerophage＇s nature immunologic response and it has a dose-dependent relationship. Meanwhile, Se has a protective function of stress to macrophage.