CYP1A1基因多态性及血清锌水平与非小细胞肺癌的关系

RELATIONSHIP BETWEEN CYP1A1 POLYMORPHISM,SERUM ZINC AND NON-SMALL CELL LUNG CANCER

[目的]探讨细胞色素P450(A)(CYP1A1)的基因多态性、血清锌水平单独作用以及联合作用与非小细胞肺癌发生危险的关系。[方法]采用病例对照研究方法,病例78例,对照78例。用限制性片断长度多态性PCR技术(PCR-RFLP)检测CYP1A1的MspI多态性,用电感耦合等离定子体发射光谱法(ICP-AES)测血清锌水平。[结果]CYP1A1的MspI多态性单独作用时与肺癌危险性关系差异无统计学意义(P﹥0.05);病例组血清锌水平显著低于对照组(P﹤0.01);以血清锌≥117.0mg/dL且CYP1A1野生型基因携带者为参照组(OR=1.00),则血清锌﹤117.0mg/dL且携带CYP1A1突变型或杂合型或野生型基因者的OR分别为5.50(P﹤0.05)、10.63(P﹤0.01)、10.08(P﹤0.01)。[结论]CYP1A1基因多态性单独作用时NSCLC发生无显著相关;血清锌水平与NSCLC发生呈负相关;CYP1A1基因多态性与血清锌联合作用时明显提高NSCLC发生的危险性,在NSCLC发生中存在协同作用。

[Objective]To study the correlation of polymorphism of CYP1A1 MspI and the level of serum zinc in independently and in combination with the risk of lung cancer. [ Methods] The ease-control study was conducted among 78 eases with the lung cancer and 78 controls. The polymorphisms of CYP1A1 were detected by PCR-RFI2, while the level of serum zinc was deternfined by ICP-AES. [Results] There was no significant difference in polymorphism frequencies of CYP1A1 MspI between the eases and the controls. The genotypes were not signifieanfly associated with the risk of hmg cancer; average level of serum zinc among the lung cancer eases was significantly lower than that of the controls （P〈0.01 ） ; comparing with those who had higher level of serum zinc （≥117.0mg/dL） with CYP1A1m1m1, the odd ratios of those who had lower serum zinc （ 〈117.0mg/dL） with CYP1A1m2m2, or CYP1A1m1m2, or CYP1A1m1m1 were 5.50 （P〈 0.05）, 10.63 （P〈0.01）, 10.08 （P 〈 0.01） respectively. [Conclusion] Polymorphism of CYP1A1 is not associated with the nsk of NSCLC, while there is a significantly inverse association between the level of serum zinc and the risk of happening NSCLC . There is a combined effect of polymorphism of CYP1A1 MspI and serum zinc on NSCLC.