反复自然流产妇女和正常妇女FXⅢ Val34Leu基因多态性的检测

DETECTION ON THE VAL34LEU POLYMORPHISM OF COAGULATION FACTOR XⅢ IN WOMEN WITH RECURRENT SPONTANEOUS ABORTION AND NORMAL WOMEN

[目的]了解深圳地区反复自然流产妇女和正常妇女凝血因子XⅢ(FXⅢ)基因Val34Leu的多态性,探讨其与反复自然流产发病的关系。[方法]应用聚合酶链反应-限制性片段长度多态性分析方法(PCR-RFLP)检测57例反复自然流产妇女和153例正常妇女的FXⅢ Val34Leu基因多态性。[结果]反复自然流产组与正常对照组均不存在FXⅢ Val34Leu的突变。[结论]深圳地区正常妇女和反复自然流产妇女FXⅢ Val34Leu的突变频率很低,FXⅢ Val34Leu的突变可能不是引起反复自然流产发病的重要原因。

[ Objective] To investigate the Val34Leu polymorphism of coagulation factor ⅩⅢ in women with recto＇rent spontaneous abortion and normal women in Shenzhen area and to explore the association with the pathogenesis of recurrent spontaneous abortion. [Methods] The Val34Lcu polymorphism of the coagulation factor ⅩⅢ was analyzed in 57 women with recurrent spontaneous abortion and 153 normal women by polymerase chain reaction and restriction fragment length polymorphism （PCR- RFLP）. [Results] There were no mutations in women with reeun＇ent spontaneous miscan＇iage and normalen. [Conclusion] The mutational frequency of the Val34Leu of the coagulation factor ⅩⅢ in women with recun-ent spontaneous abortion and normal women in Shenzhen area is very low, perhaps the Val34Leu mutation of the coagulation factor ⅩⅢ is not the important reason of the pathogenesis of recurrent spontaneous abortion.