肿瘤转移抑制基因KAI1／CD82在宫颈癌的表达及其相关性研究

STUDY ON THE EXPRESSION OF KAI1 TUMOR METASTASIS SUPPRESSOR GENE IN CERVICAL CARCINOMA AND THE CORRELATION

[目的]探讨肿瘤转移抑制基因KAI1/CD82在宫颈癌组织中的表达及其与宫颈癌侵袭和转移的相关性。[方法]采用免疫组化LsAB法检测70例浸润性宫颈癌、15例原位癌、20例正常宫颈组织以及29例转移淋巴结中KAI1/CD82蛋白的表达。[结果]KAI1/CD82在宫颈浸润癌及原位癌组织中的蛋白表达与正常宫颈上皮中的表达相比明显下调(P﹤0.05),原位癌与浸润癌中的表达差异无统计学意义(P﹥0.05);KAI1/CD82的蛋白表达与宫颈癌临床分期、病理类型、淋巴结转移、脉管浸润以及宫颈间质肌层浸润深度均无相关性(P﹥0.05);KAI1/CD82在高中分化组中的蛋白表达高于低分化组(P﹤0.05),在淋巴结转移灶中的表达与其原发灶中的表达相比差异无统计学意义(P﹥0.05)。[结论]肿瘤转移抑制基因KAI1/CD82对宫颈癌组织的恶性侵袭行为可能不是一个主要影响因素。

[Objective] To detect the expression of KAI1/CD82 metastasis suppressor gene in cervical carcinoma and the correlation with pathologic features. [Methods] Immunohistoehemistry was used to detect the expression of KAI1/CD82 gene in formalin-fixed, paraffin-embedded specimens of 20 normal cervical epithelium, 15 cervical carcinoma in situ, 70 invasive cervical carcinoma as well as 29 lymph node metastases. Correlation of expression of KAI1 gene with pathologic factors were statistically analyzed. [Results] The protein expression of KAI1/CD82 in tumor cells was down-regulation in invasive carcinoma and carcinoma in situ compared to that in controls （P〈0.05） ; the protein expression of KAI1 was not correlated with clinical stage, pathological subtype, lymph node metastasis, depth of vessel infiltration and cervical interstitium and muscular layer invasion of cervical carcinoma （P 〉 0.05）. But its expression significantly decreased in poorly differentiated tumors compared to that of well and moderately differentiated tumors. KAI1 also expressed in lymph node metastases, but there was no statistically significant difference between them with expression in situ. [Conclusion] The KAI1/CD82 metastasis suppressor gene may not be a main factor in invasion and metastasis of cervical carcinoma.