大鼠脑片体外缺血状态纹状体中一氧化氮合成酶阳性神经元的时相变化及意义

Oxygen and glucose defect induced changes with time of nitric oxide synthase positive neurons in the slices of striatum in rats.

无糖无氧人工脑脊液模拟脑缺血状态，用还原型尼克酰胺腺嘌呤二核苷酸脱氢酶反应观察大鼠离体脑片纹状体中一氧化氮合成酶阳性神经元的时相变化及神经损伤。结果显示：在缺血损伤严重的纹状体，一氧化氮合成酶阳性神经元缺血早期明显增多并深染，１ｈ后显著减少，提示纹状体的一氧化氮合成酶阳性神经元本身存在有限的抗损伤能力。

Changes of nitric oxide synthase (NOS)-positive neurons and neuronal damage of the slices of striatum were investigated with nicotinamide adenosine dinucleotide phosphate diaphorase (NADPH-d) reaction following oxygen and glucose defect in artificial cerebral spinal fluid. The results showed that the neurons in the striatum were damaged seriously, NOS-positive neurons increased early and decreased markedly one hour later. The results indicated NOS-positive neurons have a limited anti-ischemia injuny ability.