通经草叶中粉背蕨酸的分离及含量测定

Isolation and determination of alepterolic acid in the frond of Aleuritopteris argentea

目的对通经草叶中的特征成分粉背蕨酸进行分离、纯化和结构鉴定，并采用HPLC法对通经草叶中粉背蕨酸的含量进行首次测定。方法色谱柱为Betasil-C18柱（4．6mm×250mm，5μm），流动相为甲醇-体积分数为0．1％磷酸水溶液（体积比为85：15），检测波长215nm，流速1．0mL·min^-1，室温，采用外标法测定。结果粉背蕨酸质量浓度在2．475～49．50mg·L^-1内线性关系良好（r=0．9996），方法精密度（RSD）为1．6％（n=6），加佯回收率为98．2％～100．8％。结论该方法操作简便、分析速度快、重复性好、结果准确可靠，可用于通经草叶中粉背蕨酸的含量测定。

Objective To establish the method for isolation and determination of alepterolic acid in the frond of Aleuritopteris argentea. Methods The HPI.C method was performed on a Betasil-C18 column （4.6 mm × 250 mm, 5μm） at the ambient temperature, using a mixture of methanol and 0.1% phosphoric acid solution （V： V = 85：1.5） as mobile phase. The detection wavelength was set at 215 nm. The flow rate was 1.0 mL· min^-1. Results There was good linear relationship（A = 1. 078×10^7ρ- 1. 119 × 10^2, r = 0. 999 6） between the concentrations and the peak areas of alepterolic acid in the range of 2. 475 - 49.50 mg· L^-1 The recoveries were in the range of 98.2 % - 100.8 %, and the relative standard deviation was less than 1.6 % （ n = 6）. Conclusions The results indicate that this method is accurate, rapid, simple and reproducible, and thus suitable for the analysis of alepterolic acid in the frond of Aleuritopteris argentea.