巴戟天不同部位及混伪品中水晶兰苷的含量测定

Content Determination of Monotropein in the Different Parts of Morinda officinalis and Its Counterfeit Species

目的测定巴戟天及其混伪品中水晶兰苷的含量。方法采用HPLC法,色谱条件为KromasilC18柱(150mm×4.6mm,5μm),甲醇-0.4%磷酸水溶液(5:95→28.8:71.2,15min)为流动相,流速1mL/min;检测波长231nm,柱温25℃。结果除海巴戟果和羊角藤外,其他样品均含水晶兰苷,且以巴戟天和广东鸡眼藤的根、叶含量较高。结论可用巴戟天叶及其混伪品广东鸡眼藤替代巴戟天根,进行水晶兰苷的提取。

Objective To determine the content of monotropein in the different parts of Morinda officinalis and in its counterfeit species. Methods The method of HPLC was used with chromatographic conditions as follows： Kromasil C18 （ 150 mm×4. 6mm, 5 μm ） column, the mobile phase of methol - 0.4 % phosphate solution（5 ： 95→28.8 ： 71.2, 15 min）, the velocity of flow being 1 mL/min, the detection wavelength at 231 nm and column temperature being 25 ℃. Results The content of monotropein in the leaves and radix of Morinda officinalis and Morinda parvifolis is the highest. Conclusion It is reasonable to replace the root of Morinda of ficinalis with the leaves of Morinda offieinalis and Morinda parvifolis for the monotropein extraction.