甘草总黄酮提取纯化工艺研究

Extraction and Purification of Total Flavonoids in Radix Glycyrrhizae

目的研究甘草总黄酮提取分离工艺和分析方法。方法比较3种提取方法——超声波提取法、加热回流法和冷浸法对甘草总黄酮提取效果的影响;采用树脂吸附法精制甘草总黄酮并考察不同参数对提取得率和纯度的影响;采用柚皮苷直接扫描法对甘草总黄酮进行分析研究。结果超声波提取法、加热回流法和冷浸法甘草总黄酮提取率分别为5.46%、6.12%和5.04%,以加热回流法提取效果最好。树脂吸附甘草黄酮类成分主要集中在40%～60%乙醇洗脱部位。结论3种方法中以加热回流法提取甘草黄酮效果最好,树脂精制工艺考虑为纯水洗脱树脂后继以80%乙醇洗脱黄酮类成分。

Objective To study the method of extraction, purification, and analysis of total flavonoids of Radix Glycyrrhizae （TFG） . Methods The extracting effects of three different ways （ultrasonic wave, heating recirculation, and maceration） for TFG were compared. TFG was refined with resin absorption and the effects of different parameters on yield and purity were observed. Naringin scanning method was used to analyze TFG. Results TFG extracting percentage is 5.46 % by ultrasonic wave method, 6. 12 % by heating recirculation, and 5.04 % by maceration, the effect of heating recirculation being the best. In the test of resin absorption of TFG, the heating recirculation composition is mainly located in 40 % - 60 % ethanol elution parts. Conclusion The best method for extracting TFG is heating recirculation in above three methods, Elution with 80% ethanol continuously after water elution is the the optimal resin purification method for TFG.