人感染藤黄短小杆菌的分离与鉴定

Isolation and identification of Curtobacterium luteum from human

目的研究从患儿颌下淋巴结穿刺脓液中分离的纯G+棒状样杆菌的生物学地位。方法常规方法分离培养细菌,并做生化反应。提取细菌DNA,采用聚合酶链反应(PCR)扩增目的基因,琼脂糖凝胶电泳回收目的基因并测序。结果分离菌的一般生物学特性与生化反应与藤黄短小杆菌基本相同,16S rRNA基因长1 387 bp,与藤黄短小杆菌16S rRNA基因相似度达99.7%。结论通过生化反应和16S rRNA基因的序列测定确定此菌为藤黄短小杆菌,初步认为是藤黄短小杆菌的新亚型。

Objective To describe the characteristics and identify a strain of gram-positive club-shaped rod isolated from submaxillary lymphadenitis. Methods The isolated culture and biochemical test were carried on. The DNA was extracted by routine procedure. The interest gene was obtained by PCR with specific primers and purified by gel electrophoresis and sequenced. Results The biological and biochemical characteristics were similar to that of Curtobacterium luteum on the whole . The total 16S rRNA gene was 1 387 bp. The result of sequencing was closely similar（99.7%） to that of C. luteum. Conclusion On the basis of the physiological and biochemistry characteristic analysis and 16S rRNA gene sequence alignment, this strain was assigned to C. luteum and regarded as a new subtype of C. luteum initially .