大鼠慢性多重应激模型的建立

Animal Model for Chronic Multi-stress in Rats

目的:建立大鼠慢性多重应激模型,为研究应激性疾病提供实验模型。方法:健康雄性SD大鼠随机分为模型组(n=10)和对照组(n=10)。模型组采用脉冲随机变动的噪声、夜间光照、足底电击以及强迫游泳和束缚的复合刺激为应激源,对大鼠实施刺激,观察行为变化,检测心率、血压、体重增长速率、食物利用率,测定血清ACTH和皮质酮并进行分析。结果:慢性多重应激大鼠由实验之初的兴奋状态逐渐进入抑制状态,血压心率上升,体重增长缓慢,食物利用率降低,血清ACTH和皮质酮增高。结论:慢性多重应激大鼠一般行为、基本生理体征和下丘脑-垂体-肾上腺轴(HPA)功能发生改变,应激反应处于持续亢奋状态,该模型是较稳定较理想的应激模型。

Objective： To establish animal model for chronic multi-stress in rats, to provide a platform for study the disease resulted from stress. Method： SD rats were administered with different multi-stress, such as noise, light, electric foot shock and swimming combined with immobilization. The behavioral were recorded and analyzed, the heart rate, the blood pressure and the rate of food utilization were examined, and the level of the serum ACTH and hydrocortisone were determined to evaluate the model. Result： There were obvious behavior changes in the rats of model group. Compared with control group, Heart rate, blood pressure and the level of serum ACTH and corticosterone increased significantly, the rate of Food utilization decreased significantly. Conclusion： In this study, the activity of HPA axis enhanced in the rats of model group, the animal model for chronic multi-stress was established successfully.