摘要
目的:研究胶原蛋白多肽铬螯合物对小鼠四氧嘧啶致肝损伤在形态结构方面的保护作用。方法:30只昆明小鼠随机分为正常对照组、四氧嘧啶模型组及胶原蛋白多肽铬螯合物(CPCC)组。胶铬组灌胃(Cr^(3+)40μg/kg BW)28d,正常组和模型组给予等体积蒸馏水。腹腔注射四氧嘧啶造模,3d后观察肝脏形态学变化、测定肝指数并分析肝脏可溶性蛋白的变化。结果:CPCC处理使四氧嘧啶处理后小鼠肝脏肿大、肝小叶粘连现象明显改善;肝指数降低;减轻肝细胞变形、肝窦淤血、炎症细胞浸润及肝细胞空泡样变、血管内皮细胞损伤,核变性程度。同时CPCC能有效防止四氧嘧啶所致小鼠肝脏可溶性蛋白的变化。结论:CPCC对四氧嘧啶致小鼠肝脏损伤具有明显的保护作用。
Objective： To study the protective effect of collagen peptide-chromium chelate （CPCC） on liver injury in alloxan-treated mice. Methods： Thirty kunming mice were divided into control group, alloxan-treated group and CPCC group. CPCC group treated with CPCC （Cr^3＋40μg/（kg BW .d） ） orally for 28 days. Other two groups were treated by distilled water. After 3 days of alloxan treatment, morphological alterations, liver index and liver proteins were examined. Results： CPCC treatment was found to effectively attenuate the degenerative changes observed in alloxan-induced animals, such as hypertrophy of liver, adhesion phenomenon between adjacent hepatic lobules, crimpling and partial necrosis of hepatocytes, inflammatory cell infiltration, vacuolation, endothelial cell injury and the nuclear damage etc. Conclusions： The results of morphological investigations provided direct evidences for the protective effect of chromium against the liver injury in alloxan-treated animals.
出处
《现代生物医学进展》
CAS
2008年第1期44-46,共3页
Progress in Modern Biomedicine
基金
天津市自然科学基金(No.013617111)
