急性髓性白血病SCID小鼠模型的建立及鉴定

Establishment and Evaluation of a Murine Model of AML

目的:探讨建立急性髓性白血病小鼠模型,并对模型建立过程进行观察、分析和鉴定。方法:重症联合免疫缺陷小鼠(SCID)建立HL-60细胞白血病动物模型,观察SCID小鼠一般情况,监测小鼠外周血HL-60细胞、白细胞、红细胞、血小板的变化,用流式细胞术、骨髓染色体检查等检测小鼠骨髓,濒死或死亡小鼠行组织病理学检查。结果:接种后3-5周,外周血白细胞上升,外周HL-60细胞占非红系有核细胞比例为3%-11%。骨髓HL-60细胞占非红系有核细胞中比例为40%-58%,骨髓细胞中CD33抗原阳性率8.18%-24.45%。小鼠骨髓细胞中有中、亚中部着丝点染色体。结论:HL-60细胞静脉接种SCID小鼠,白血病发病率为100%。其发病症状及组织浸润与人类白血病相类似,可用于研究白血病增殖、分化等机制,是探索新的白血病治疗手段及判断患者预后的重要载体。

Objective： To establish and evaluate a mice model of AML. Methods： We use SCID mice to establish human acute promyelocytic leukemia HL-60 model. Physiological status of the mice was monitored, as well as the WBC, RBC, PLT, HL-60 cell count in the peripheral blood. The histology assay was performed in the dying and dead mice. Leukemic cells were detected by chromosome karyotype analysis and flow cytometry analysis in marrow. Results： 3 to 5 weeks after inoculation, peripheral blood neutrophils increased and the proportion of HL-60 cells in nucleated cells has come to 3%-11% in peripheral blood and 40%-58% in marrow. CD33＋ cells take 8.18%-24.45% of the marrow cells. And metacentric and submetacentric chromosomes were observed in SCID mice. Conclusion： Intravenous （i.v.） inoculation of HL-60 cells lead to leukemia in SICD mice, and which has similar symptoms and infiltration of human being. SCID mouse-human leukemia model is an important and useful tool for studies on proliferation, differentiation and modulation of leukemic cells, as well as studies of pathogenesis, clinical diagnosis, therapy and assessment of prognosis of leukemia patients.