超声提取-分光光度法测定远志总皂苷的含量

Quantitative Determination of Total Saponins in Polygalae by Spectrophotometry

目的:超声提取远志总皂苷,并用分光光度计进行总皂苷含量的测定。方法:超声提取远志总皂苷,以远志皂苷元为对照品,香草醛-冰醋酸-高氯酸为显色剂,采用分光光度法在585nm处测定远志总皂甙含量。结果:对照品在28～63μg范围内线性良好(r^2=0.9984),平均回收率为100.45%,RSD=1.59%(n=5)。超声提取和热回流提取的远志总皂苷含量分别为1.63%,1.50%。结论:超声提取法用时短,提取率高,能达到比传统回流法更理想的效果。分光光度法测定含量简便、快速、准确、可靠。

Objective： To discuss the use of spectrophtotmetry in the quantitative determination of total saponins in Polygala. Methods： The total saponins in Polygala tenuifoli were extracted by ultrasonic wave. Senegenin was used as standard control and vanillin-perchloric acid was used as chromogenic reagent. The detection wave length was 585 nm. Results： Absorbance shows linear relationship to the total saponins concentration in the range of 28-63μg at 585nm. The regression equation of concentration was obtained： A =0.0079 C ＋0.0689 （μg） with a correlation coefficient of r^2=0.9984. The average recovery of standard control was 100.45%, and the relative standard deviation （RSD） of precision test was 1.59% （n=5）. The concentration of total saponins in Polygala extracted by ultrasonic wave and circumfluence was 1.63% and 1.50% respectively. Conclusion： Ultrasonic wave extraction is more efficient than the circumfluence extraction. Spectrophtotmetry is simple, rapid, sensitive and accurate for the quantitative determination of total saponins in Polygala.