监测反复自然流产者血清HCMVIgM抗体及其绒毛组织DNA的研究

A study on serum HCMV IgM antibody and on the DNA of chorionic tissue in the repeated spontaneous abortion patients.

采用酶联免疫吸附测定法（ＥＬＩＳＡ）对８５例反复自然流产（ＲＳＡ）妇女及１０７例无流产史早孕妇女的血清进行ＨＣＭＶＩｇＭ、ＨＣＭＶＩｇＧ检测，应用ＰＣＲ技术检测部分绒毛组织ＤＮＡ，直接法制备绒毛细胞染色体。结果显示：ＲＳＡ组ＨＣＭＶＩｇＭ阳性率（５８．８２％），明显高于对照组（１５．８９％）（Ｐ＜０．０１）。说明孕妇原发性ＨＣＭＶ感染与ＲＳＡ有相关性；ＨＣＭＶＩｇＭ阳性与流产次数有密切关系；ＨＣＭＶ可以通过胎盘屏障感染胎儿。

Objective:To study 85 women with repeated spontaneous abortion(RSA) and 107 normal early pregnant women.Design:Using ELISA method to detect serum HCMV-IgM,HCMV-IgG and using PCR technique to examine chorionic tissue DNA by direct method to prepare chromosome in chorionic cell.Results:HCMV-IgM positive rate in RSA group (58.82%) is significantly higher than that in control group(15.89%)(P<0.01).Conclusion:RSA is correlated with primary infectin of HCMV in pregnant women.There is close correlation between the HCMV-IgM positive and the number of abortion.IgG in HCMV-IgM positive women can pass through placental-barrier to infect fetus and cause abortion. \ \