脉冲场凝胶电泳技术在猪链球菌分型中的应用

Application of Pulsed Field Gel Electrophoresis in Classification of Streptococcus suis

脉冲场凝胶电泳是一种可用于分离20 kb至10 Mb大分子质量DNA的新型凝胶电泳技术。该法应用于猪链球菌的分子分型,通过观察电泳条带的差异,为确定菌株之间的亲缘关系,分析基因型和临床症状之间的关系等方面提供了可靠的技术手段。文章介绍了脉冲场凝胶电泳技术的应用状况及猪链球菌的分型现状,并阐述了该技术在猪链球菌分子分型研究方面的应用及发展前景。

Pulsed Field Gel Electrophoresis （PFGE） is a new technique of gel electrophoresis which can be used to separate large DNA molecules ranging from 20 kb to 10 Mb. It has been widely applied to the karyotype analysis and karyotype polymorphism for various bacterium. It applies in the genetic classification of Streptococcus suis to reveal discrimination among different strains and analyses the relation between genotype and clinical symptom through observing the patterns of restriction fragment. In this paper, based on the introduction of the principle of PFGE, the classification of Streptococcus suis, the application and development of PFGE in the research of genetic classification of Streptococcus suis were discussed.