生存素、p27和PTEN在肝细胞癌中的表达及临床病理意义

Expression of survivin, p27 and PTEN in hepatocellular carcinoma and their clinical significances

目的探讨生存素蛋白、生存素mRNA、p27蛋白、p27mRNA和第10号染色体同源丢失性磷酸酶张力蛋白基因（PTEN）蛋白在肝细胞癌（HCC）中的表达及其临床病理意义。方法自制组织芯片，采用免疫组织化学和原位杂交法检测生存素蛋白、p27蛋白、PTEN蛋白和生存素mRNA、p27mRNA在141份肝细胞癌、128份癌旁肝组织、97份远癌肝组织及17份正常肝组织中的表达，探讨各指标的关系并建立预测肝癌发生的模型。结果肝癌组中生存素蛋白（Ridit值的95％甜为0．689±0．048，P〈0．01）、生存素mRNA（Ridit值的95％甜为0．690±0．049，P〈0．01）和p27蛋白（Ridit值的95％a为0．556±0．053，P〈0．05）表达明显增高，PTEN（Ridit值的95％a为0．282±0．048）表达明显下降（P〈0．01）；肝癌中生存素的表达与p27、PTEN表达均显著相关；生存素mRNA、p27蛋白和PTEN蛋白对判断肝癌发生与否有重要意义。结论生存素mRNA、p27蛋白的表达上调和PTEN蛋白的表达下调可作为判断肝癌发生的有价值指标。

Objective To detect the expression of survivin protein, survivin mRNA, p27 protein, p27 mRNA and PTEN protein in hepatocellular carcinomas （HCC） and their clinical significances. Methods Tissue microarrays were constructed. The expression of survivin protein, p27 protein and PTEN protein were evaluated by immunohistochemical methods and in expression of survivin mRNA and p27 mRNA were evalu- ated by in stiu hybridization respectively in tumor tissues from 141 HCC patients, 128 samples of paracarcinoma liver tissues, 97 liver tissues far from the carcinomas and normal liver tissues from non HCC patients. The relationship of survivin, p27 and PTEN were investigated and a prediction model of HCC was constructed.Results The expressions of survivin protein （Ridit 95% CI = 0.689 ± 0.048, P 〈 0.01）, survivin mRNA （Ridit 95% CI = 0.690 ± 0.049, P〈 0.01） and p27 protein （Ridit 95% CI = 0.556 ＋_ 0.053, P 〈 0.05） in HCC tissues were significantly increased, while the expression of PTEN protein （Ridit 95% CI = 0.282± 0.048） in HCC tissues was significantly reduced （P 〈 0.01）. Conclusion Overexpressions of survivin mRNA and p27 protein and reduced expression of PTEN protein might be a valuable marker to predict the presence of HCC.