两种脱矿体系制备人工龋损的病理学比较

Pathological Comparison of Two Type of Demineralization Solutions on the Production of Initial Artificial Enamel Carious Lesion

目的评价两种不同脱矿体系在人牙釉质与牛牙釉质形成人工龋样损害的情况,确定模拟早期龋实验研究的脱矿系统。方法两种脱矿体系:部分饱和酸缓冲溶液和酸性凝胶溶液,分别在人牙釉质与牛牙釉质制备人工早期釉质龋损,采用偏振光显微镜和扫描电子显微镜观察实验人工龋的组织病理学变化。结果部分饱和的酸缓冲体系制备的人工龋损都具有完整的表层和表层下脱矿,牛牙釉质除了脱矿深度大于人牙釉质外,其它病理学特点都类似于人牙釉质,与早期自然龋损相似。而凝胶液脱矿体系形成的人工龋损不管是人牙还是牛牙都没有明显的表层结构,实验牙表面多出现被腐蚀或溶解。结论部分饱和的酸缓冲液脱矿系统形成的人工龋损病理学变化更接近于自然龋;与人牙具有相似化学组成的牛牙很适合代替人牙来检测脱矿和再矿化的效果。

Objective To determine the effect of two type of demineralization solutions on the production of artificial carious lesion in bovine enamel and human enamel in vitro. Methods A partially saturated acidic buffer solution and an acidifed hydroxyethylcellulose gel system were used as decalcifying media for the preparation of initial artificial carious lesion in bovine enamel and human enamel. After demineralization, these enamel specimens was observed by microscope and scaning electron microscope and compared in histological pathology. Results The results indicated all specimens in a partially saturated acidic buffer solution could morphologically form subsurface demineralization, which were the same as the early smooth enamel caries. Lesions in bovine teeth were pathologically similar to those in human teeth, except that the lesions depth was deeper than those in human teeth. However, In gel, regular subsurface lesions were hardly produced in all specimens. They were either eroded or dissolved. Conclusion It was concluded that caries-like lesions could be consistently produced in bovine enamel with a partially saturated acidic buffer solution. So this demineralization system can be use for reproducible preparation of artificial carious lesions in dental enamel.