新生牛肝活性肽对小鼠的免疫增强作用

Immunologic Enhancement of Newborn Bovine Liver Active Peptide in Mice

目的观察新生牛肝活性肽对小鼠免疫功能的调节作用。方法采用清洁级ICR小鼠,分别经口给予2.08、4.16和12.48ml/kg3个剂量的新生牛肝活性肽,以生理盐水为对照组,通过淋巴细胞转化试验、小鼠迟发型变态反应试验、血清溶血素测定、抗体生成细胞检测、小鼠碳廓清试验、腹腔巨噬细胞吞噬鸡红细胞试验及NK细胞活性测定来评价其对小鼠的免疫增强作用。结果3个剂量组均能促进淋巴细胞增殖,中、高剂量组能增强小鼠迟发型变态反应强度和小鼠血清溶血素作用,提高溶血空斑数及NK细胞活性,但对小鼠的碳廓清能力及腹腔巨噬细胞的吞噬能力无明显影响。结论新生牛肝活性肽能增强小鼠的免疫功能。

Objective To observe the regulatory effect of newborn bovine liver active peptide immunologic funetion of mice.Methads Divide ICR mice into 3 groups and treat with newborn bovine hver active peptide by oral route at dosages of 2.08（low）,4.16（mederate） and 12.48 ml/kg bodyweight（high）respectively,once a day for 30 d,using sterile physiological saline as control. Evaluate the regulatory effect of newborn bovine liver active peptide by splenic lymphocyte transrormation test, delayed hypersensitivity test, determination of serum hemolysin and antibody-producing cells, carbon clearance test, macrophage phagocytic function test and determination of NK cell activity. Results All the newbom bovine liver active peptide at various dosages promoted the proliferation of splenic lymphocytes, and those at moderate and high dosages enhanced the delayed hypersensitivity, increased the hemolysin level in sere, the number of antibody-producing cells and the activity of NK ceils. However, it showed no significant effect on carbon clearance rate and macrophage phagocytic function of mice. Conclusion Newborn bovine liver active peptide enhanced the immunologic function of mice.