1Abrahante JE, Daul AL, Li M, et al.The caenorhabditis elegans hunchback-like gene lin-57/hbl-1 controls developmental time and is regulated by microRNAs. Dev CEU,2003,4(5):625-637.
2Dykxhoom DM, Novina CDandSharp PA. Killing the messenger: short RNAs thai silence gene expression. Nat Rev Mol Cell Biol,2003,4(6):457-467.
3Lee SK,Dykxhoom DM,Kumar P, et al.Lentiviral delivery of short hairpin RNAs protects CD4 T cells from multiple clades and primary isolates of HIV. Blood,2005,106(3) :818-826.
4Zamore PD, Hutvagner G. A microRNA in a multiple-turnover RNAi enzyme complex.Science ,2002,297(5589) :2056-2060.
5Ambion.Design a better siRNA.2003 cited; Available from: htpp://www.ambion. com/techlib/tn/104/5.html. 2003 sep.
6Xiao H, Wang Y, Liu D. Microarray analysis and RNA silencing link fra-1 to cd44 and c-met expression in mesotherlioma. Caner Res, 2003, 63(13):353945-353954.
8Ambion.Five ways to produce siRNA.2003 cited; Available from: http:www. ambion.com/techlib/tn/103/2.html.
9Saxena S,Jonsson ZO, Dutta A.Small RNAs with impedect match to endogenous mRNA repress translation. Implications for off-target activity of small inhibitory RNA in mammalian cells. J Biol Churn,2003,278(45):44312-44319.
10Jackson AL, Bartz SR,Scheher J, et al. Expression profiling reveals off- target gene regulation by RNAi. Nat Biotechnol,2003,21 (6):635-637.
2[2]McManus MT, Sharp PA. Gene silencing in mammals by small interfering RNAs[J]. Nat Rev Genet,2002,3(10): 737~747.
3[3]Fagard M, Boutet S, Morel JB, et al. AGO1, QDE-2, and RDE-1 are related proteins required for post-transcriptional gene silencing in plants, quelling in fungi, and RNA interference in animals[J]. PNAS.2000, 97(21): 11650~11654.
4[4]Parrish S, Fire A. Distinct roles for RDE-1 and RDE-4 during RNA interference in Caenorhabditis elegans[J].RNA,2001, 7:1397~1402.
5[5]Hamilton AJ, Baulcombe DC. A species of small antisense RNA in posttranscriptional gene silencing in plants[J]. Science, 1999,286: 950~952.
6[6]Hammond S, Bernstein E, Beach D, et al. An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells[J]. Nature, 2000,404: 293~298.
7[7]Zamore PD, Tuschl T, Sharp PA, et al. RNAi: Double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals[J]. Cell, 2000,101: 25~33.
8[8]Elbashir SM, Harborth J, Lendeckel W, et al. Duplexes of 21-nucleotide RNAs mediate RNA interference in mammalian cell culture [J]. Nature,2001, 411: 494~498.
9[9]Elbashir SM, Lendeckel W, Tuschl T. RNA interference is mediated by 21 and 22 nt RNAs [J]. Genes & Dev, 2001,15: 188~200.
10[10]Elbashir SM, Martinez J, Patkaniowska A, et al. Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate[J]. EMBO J,2001,20: 6877~6888.