含邻酚结构化合物氧化反应中自由基的检测

A Study on the Free Radicals in the Oxidation of Some Compounds with o Hydroxyphenyl Group

用电子自旋共振技术研究了水溶液中邻苯二酚、ＤＯＰＡ和槲皮素等化合物与Ｏ２－反应中产生的活性自由基中间体，根据所观察别的ＥＳＲ谱的超精细结构常数可以推断它们都是邻半醌负离子基，在自旋捕获实验中发现无水乙腈中碱性条件下这些化合物与Ｏ２反应时会产生Ｏ２－，这表明在某些重要的生物抗氧化剂的抗氧化过程中，可能产生新的活性氧．

This paper studies the free radicals yielded in the reactions between superoxide anion radical and catechol(or DOPA,quercetin,etc.)in aqueous solutions by ESR technique.It can be inferred from the hfs constants of ESR spectra observed that these free radicals are all o semiqunoid anion radicals.In spin trapping experiments,it was also found that the superoxide anion radicals were get from the reactions of O 2 with some compounds with o hydroxyphenyl group in acetonitrile,which evidently shows that new active oxygen species might be produced during the antioxygenic process of some important biological antioxidants.