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可溶性Jagged1/Fc对DC-T细胞共培养中细胞因子表达的影响 被引量：4

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摘要 目的:研究可溶性Jagged1/Fc对DC-T细胞共培养体系中TGF-β1、IL-10、IL-4、IL-2、IFN-γ及TNF-α表达水平的影响。方法:应用IL-4和GM-CSF诱导培养小鼠骨髓细胞,流式细胞术检测髓系树突状细胞(DC)分化标志CD11c;将等量的淋巴细胞与之共培养,ELISA检测共培养上清TGF-β1的含量,Luminex100检测IL-10、IL-4、IL-2、IFN-γ和TNF-α的含量。结果:可溶性Jagged1/Fc处理组TGF-β1和IL-10的水平与DAPT对照组之间无统计学意义(P>0.05),而IL-4、IL-2、IFN-γ和TNF-α的水平升高(P<0.05)。结论:Jagged1/Fc能促进DC-T细胞相互作用导致IL-4、IL-2、IFN-γ和TNF-α的水平上调。
作者 王炯坤 邢飞跃 季煜华 方志远 郭中锋 潘山
机构地区 暨南大学组织移植与免疫研究中心
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2008年第2期186-188,共3页 Chinese Journal of Cellular and Molecular Immunology
基金 国家自然科学基金资助项目(30471635) 广东省自然科学基金资助项目(04010451 5006033)
关键词 Jagged1/Fc 细胞因子 树突状细胞 T细胞
分类号 R392.12 [医药卫生—免疫学]
  • 相关文献

参考文献12

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二级参考文献21

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共引文献2

同被引文献30

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引证文献4

二级引证文献18

细胞与分子免疫学杂志
2008年 第2期

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