病毒性心肌炎患儿血清巨噬细胞移动抑制因子、肿瘤坏死因子-α、白细胞介素-1β表达及其意义

Expressions of Macrophage Migration Inhibitory Factor,Tumor Necrosis Factor-α and Interleukin-1β in Serum of Children with Viral Myocarditis

目的探讨巨噬细胞移动抑制因子(MIF)、TNF-α、IL-1β在病毒性心肌炎(VM)患儿血清中表达的意义。方法收集衡阳市中心医院和南华大学第一附属医院收治的30例急性期和20例恢复期VM患儿及30例健康儿童血清,采用ELISA测定其血清MIF、TNF-α、IL-1β水平,采用日立7180型全自动生化分析仪检测磷酸肌酸激酶同工酶(CK-MB),并与30例健康儿童作对照。结果VM急性期患儿血清MIF[(136.7±32.2)ng/L]、TNF-α[(247.6±48.2)ng/L]、IL-1β[(19.7±4.4)ng/L]及CK-MB[(34.2±9.7)U/L]水平均明显高于恢复期及健康对照组,有显著性差异(F=17.2,21.4,13.5,14.1Pa<0.01),且血清MIF、TNF-α、LI-1β及CK-MB水平随病情加重而增高,MIF与CK-MB,TNF-α与CK-MB、IL-1β与CK-MB均呈正相关(r=0.68,0.82,0.73Pa<0.05);VM恢复期MIF[(41.8±8.2)ng/L]、TNF-α[(67.5±12.1)ng/L]、IL-1β[(6.4±1.2)ng/L]及CK-MB[(12.6±4.1)U/L]水平与健康对照组比较,无显著性差异(Pa>0.05),且MIF、TNF-α、IL-1β与CK-MB无相关性(Pa>0.05)。结论VM患儿存在细胞免疫功能紊乱,MIF、TNF-α、IL-1β可能参与VM发病过程,并可作为病情判断及疗效的观察指标之一。

Objective To explore the expression levels of maerophage migration inhibitory factor （MIF） ,tumor necrosis factor -α（TNF - α） and interleukin - 1 β （ IL - 1 β） in serum of children with viral myoearditis（VM）. Methods The serum levels of MIF, TNF - α and IL - 1 β were detected by enzyme linked immunosorbent assay（ELISA） in 50 children with VM came from central hospital of hengyang and the first affiliated hospital of nanhua university, and 30 normal heahhy children. CK - MB were measured by fully automated clinical chemistry analyzer. Results The serum levels of MIF[ （136.7±32.2） ng/L] ,TNF-α[ （247.6 ±48.2） ng/L] ,IL- 1β[ （19.7 ±4.4） ng/L] and CK - MB [ （34.2 ± 9.7） U/L] of VM group in acute stage were significantly higher than those of VM group in recovery stage and heahh control group （Pa 〈0.01） ,and there was positive correlation between MIF,TNF-α,IL-1β and CK-MB（r=0.68,0.82,0.73 P 〈0.05）. But there were no significant difference severity of the disease in the serum levels of MIF[ （41.8 ± 9.2） ng/L] ,TNF-α [ （67.5 ± 12.1 ） ng/L], IL-1β [ （6.4 ± 1.2） ng/L] and CK-MB [ （12.6 ± 4.1 ） U/L] between VM group in recovery stage and health control group（P 〉 0.05） ,and there was no correlation between MIF,TNF-α, IL- 1β and CK- MB in recovery stage（P 〉 0.05 ）. Conclusions It suggests that immune dysfunction exists in child patients with VM, MIF,TNF - α and IL - 1 β may play a role in the VM pathogenesis,and they can be a new indicator for VM.