PTX阻断溶血磷脂酸对大鼠胚胎神经干细胞的促增殖作用(英文)

PTX blocks the promotive action of LPA on proliferation of embryonic neural stem cells in rats

目的观察溶血磷脂酸(lysophosphatidic acid,LPA)对大鼠胚胎神经干细胞(neural stemcells,NSCs)的增殖的影响以及百日咳毒素(pertussis toxin,PTX)对LPA作用的影响。方法体外培养神经干细胞,对生成的神经干细胞球进行计数显示增殖情况。结果在特殊的无血清培养液中加入低浓度LPA(0.01-1.0μmol/L)后,神经干细胞球数量呈剂量依赖性增加,表明LPA对NSCs有显著的促增殖作用;②培养液中同时加入LPA和PTX后,神经干细胞球计数显示LPA引起的NSCs的增殖被抑制了98%。结论PTX阻断了LPA对大鼠胚胎神经干细胞的促增殖作用,从而推测LPA的促增殖作用可能主要通过PTX敏感的Gi-Ras-Raf-MAPK信号转导途径实现。

Objective To study the effect of lysophosphatidic acid（LPA）on the proliferation of rat embryonic neural stem cells （NSCs） in vitro and the effect of pertussis toxin （PTX） on LPA-induced promotive proliferation of NSCs. Methods NSCs were cultured in vitro, and neurospheres were counted. Results ①Lower concentrations of LPA （0.01-1.0μmol/L） dose-dependently increased the numbers of neurospheres cultured in specific serum-free media, which indicated a significant promotive action of LPA on the proliferation of NSCs. ②After NSCs were treated by 1.0 μmol/L LPA combined with PTX, 98% of the LPA-induced proliferation was blocked. Conclusion These results suggest that the promotive effect LPA might be brought about mainly by the PTXsensitive Gi-Ras-Raf-MAPK pathway rather than the PTX-insensitive ones.