食品添加剂亚硫酸钠对HL-7702肝细胞的毒性作用

The toxicity of sodium sulfite additives on HL-7702 hepatocyte

目的探讨亚硫酸钠(Na2SO3)对HL-7702正常人肝细胞的损伤作用。方法经不同浓度(分别为0.039,0.156,0.625,2.5mmol/L)的Na2SO3染毒24h后,采用MTT法观察Na2SO3对肝细胞活性的抑制作用和通过测定肝细胞培养上清中乳酸脱氢酶(LDH)活性来反映其对肝细胞膜的损害。结果①随着Na2SO3染毒剂量的增加,OD值逐渐降低,肝细胞的活性抑制率逐渐升高;其中0.625mmol/L和2.5mmol/L染毒组与阴性对照组相比有统计学意义(P<0.05)。②肝细胞培养上清中的LDH随染毒剂量的增加而呈上升趋势,与对照组相比有显著性差异(P<0.05),并且有一定的剂量反应关系(r=0.841,P<0.01)。结论①Na2SO3对肝细胞的活性有一定的抑制作用;②Na2SO3染毒24h后使肝细胞培养上清中LDH活性增高,提示其对肝细胞膜可能有一定的损伤作用。

Objective To investigate the toxicity of sodium sulfite （Na2SO3） on HL-7702 hepatocyte. Methods The HL-7702 cells were exposed to different concentrations of Na2SO3（0. 039, 0. 156, 0. 625, 2.5 mmol/L） for 24 h. The cell viability inhibition was observed by MTT assay and the activities of lactate dehydrogenase（LDH） in the culture supernatant were determined to assess the hepatic cell membrane damage. Results ①Compared with negative control group, OD values in 0. 625 mmol/L Na2SO3 and 2.5 mmol/L Na2SO3 treated groups decreased significantly, while the inhibition rotes of the cell viability increased significantly （P〈 0.05）.②Compared with the negative control group, the activities of LDH in supernatant of HL-7702 increased significantly as the concentration of Na2SO3 increased （ P 〈 0.05）, and there was in a concentration-dependent manner （ r = 0.841, P 〈 0.01）. Condusion （DNa2SO3 can inhibit the activity of HL-7702 to some degree. ③The activities of LDH in the hepatocyte cultural supernatant increase after 24 h exposure to sodium sulfite, which indicates that sodium sulfite may induce some damages on HL-7702 hepatocyte membrane.