荧光定量PCR技术检测流产组织中人细小病毒B19的感染

Determination of human parvovirus B19(HPV B19) in abortion tissues by fluorescent quantitative PCR

目的比较荧光定量PCR(FQ-PCR)与PCR在检测自然流产组织人细小病毒B19(HPV B19)感染临床应用中的优劣。方法收集自然流产病例41例以FQ-PCR与PCR分别进行流产组织的HPV B19 DNA检测。结果FQ-PCR HPV B19 DNA的阳性检出率为51.20%,PCR HPV B19 DNA的阳性检出率为36.56%,两种方法的阳性检出率经统计学检验具有统计学差异(P<0.05)。结论FQ-PCR的灵敏性较PCR高,能更加清楚地了解HPV B19在体内的存在状态。

Objective To compare the advantages and disadvantages of fluorescent quantitative PIER （FQ-PCR） and polymerase chain reaction （PCR） in determining human parvovirus B19 （HPV B19） in abortion tissues. Methods Tested tissues from 41 cases of unexplained spontaneous abortion were studied using FQ-PCR and PCR assay for HPV B19. Results The prevalence of HPV B19 DNA was 51.20 % by FQ-PCR and 36.56% by PCR in the sera of abortion women, respectively. There was significant difference between them（P 〈 0.05）. Conclusion The results suggest that FQ-PCR is more sensitive and efficient than traditional PCR for deteting HPV B19 from abortion tissues.