PSA及PSA mRNA在前列腺癌诊断中的价值

Diagnostic value of PSA and PSA mRNA in prostate cancer

目的评价前列腺特异性抗原(PSA)及PSA mRNA对前列腺癌的诊断价值。方法选取老年男性前列腺癌患者53例作为受试对象,同时选取前列腺增生患者30例作为对照组,采用ELISA法检测血清PSA水平,采用反转录聚合酶链反应检测其mRNA的表达。结果前列腺增生患者总PSA小于10ng/ml,而前列腺癌患者总PSA大于10ng/ml。当PSA小于4ng/ml和大于10ng/ml时,PSA和PSA mRNA对前列腺癌的诊断与金标准相比,无统计学意义(P>0.05)。当PSA在诊断灰区4-10ng/ml之间时,PSA mRNA与金标准相比,无统计学意义,而PSA低于金标准(P<0.05)。结论血清PSA及PSA mRNA的检测对前列腺癌的诊断具有极其重要的作用,检测PSA的同时进行PSA mRNA的检测,有助于前列腺癌的诊断,尤其对PSA在诊断灰区的前列腺癌患者。

Objective To evaluate the diagnostic value of PSA and PSA mRNA for prostate cancer. Methods PSA mRNA and PSA were determined by RT-PCR and ELISA in 53 patients with prostate cancer and 30 controls with benign prostatic hyperplasia （BPH）. Results The total PSA level in BPH was always less than l0 ng/ml,while in patients with prostate cancer was always higher than 10 ng/ml, When PSA was lower than 4 ng/ml or more than 10 ng/ml, there was no significant difference between PSA, PSA mRNA and pathologic diagnosis for prostate cancer. When PSA was 4 - 10 ng/ml, compared with pathologic diagnosis, PSA mRNA had no significant difference in diagnosis of prostate cancer （P〉0, 05）, while PSA was significantly lower （P〈0.05）, Conclusion Serum PSA and PSA mRNA could be useful markers for diagnosing prostate cancer. Combination of PSA and PSA mR- NA could be helpful to diagnose prostate cancer, especially for prostate cancer with PSA level between the gray zones.