川崎病患者血清MIF和IL-6的检测及临床意义

Serum levels of macrophage migration inhibitory factor and interleukin-6 in patients with Kawasaki disease and their clinical significance

目的探讨川崎病患者不同疾病时期血清巨噬细胞迁移抑制因子(macrophage migrationinhibitoryfactor,MIF)和白细胞介素-6(interleukin-6,IL-6)的水平、二者间的相互关系及其可能的临床意义。方法川崎病患者52例、其他热性对照42例、正常对照30例,采用酶联免疫吸附试验(enzyme-linkedi mmunosorbent assay,ELISA)检测三组血清MIF和IL-6水平,其中川崎病患者分别于急性期(发病第0-2周)与亚急性期(发病第2-4周)各检测一次。结果川崎病患者血清MIF和IL-6水平明显高于正常对照组和其他热性对照组,尤以急性期最为显著(P<0.01)。川崎病同一个体不同病程期间,血清MIF和IL-6水平有很好的相关性,特别是急性期患儿二者呈正相关(r=0.87,P=0.017)。结论急性期川崎病患儿血清MIF和IL-6水平明显升高,且二者呈正相关,可望作为疾病急性期的一个判断指标。

Objective To measure serum levels of macrophage migration inhibitory factor （MIF） and interlerkin-6 （IL-6） in patients with Kawasaki disease （KD）, and explore their clinical effects. Methods Serum levels of MIF and IL-6 in 52 patients with KD, 30 normal healthy subjects, and 42 febrile control subjects were determined by enzyme-linked inmmunosorbent assay （ELISA）. Results Serum levels of MIF and IL-6 in acute stage of KD increased significantly compared with those in subacute stage （P〈 0.01 ）, normal controls （P 〈 0.01）, and febrile controls （P = 0.01 ）. And there was also a positive correlation between serum levels of MIF and IL-6 in patents with KD, especially in acute stage （ r = 0.87, P = 0. 017）. Conclusion MIF and IL-6 may be useful markers for studying the pathogenesis and treatment of KD in acute stage of KD.