患者输血前后血液一氧化氮水平变化

The changes of patients' blood concentrations of nitric oxide after blood transfusion

目的为了了解输血对病人血液中一氧化氮浓度的影响,给临床输血提供一些参考。方法取我院因贫血入院病人标本,每位病人输红细胞都在4单位以上。分别在输血前及在输血完毕后2小时内留取血浆标本于-20℃保存,然后用NO检测试剂盒测定各标本及标准品的OD值,并计算的各标本的NO含量。结果病人在输血后血浆一氧化氮浓度有明显升高。根据统计学处理显示,输血后血浆中一氧化氮的浓度与输血前比较有显著性的变化。结论病人输血后NO浓度升高的原因,可能与红细胞作为一氧化氮的储存和运输载体有关,受血者会产生一种免疫应答。存在于巨噬细胞内的iNOS在机体免疫应答时出现表达合成NO。这可能是引起受血者血液一氧化氮水平升高的另一个原因。输血导致的一氧化氮浓度的升高可能会增加了对机体免疫系统的抑制。影响大出血病人及创伤性脑损伤病人的预后。

Objective To understand the blood transfusion on a patient＇ s blood concentrations of nitric oxide, and provide some reference to Clinical transfusion.Methods The blood samples came from anemia petients.Who transfused more than four RBC units blood. In the blood transfusion before and after two hours. The collecting plasma samples were kept at - 20℃. The OD value of standard specimen and other samples was testd by the Nitric Oxide detection kit, then was calculated the various specimens of the concentrations of Nitric Oxide. Results The patients＇ plasma concentrantions of Nitric Oxide increased significantly after blood transfusion. According to statistical analysis, it shows that plasma concentration of Nitric Oxide has significant change before blood transfusion. Conclusion The patients＇concentration of Nitric Oxide elevated, perhaps because RBC as a Nitric Oxide storage and transport of the cartier. Acceptors produce an immune response, iNOS in the macrophages express and synthese Nitric Oxide in the immune response. This may be another reason for recipients of blood Nitric Oxide levels rise. Transfusion causeing a higher concentration of Nitric Oxide may increase the immune system suppressed, and has a bad effect on the bleeding patients and the traumatic brain injury patient.