摘要
对市售氯霉素原料经提纯后研制兽药标准物质,采用熔点、红外和质谱法对提纯后氯霉素样品进行定性鉴定,采用高效液相色谱法进行定量分析。探讨了高效液相色谱法分析检测氯霉素兽药的分离与检测条件。试验发现氯霉素在甲醇溶剂中不稳定,在乙腈溶剂中稳定性提高。采用多家实验室联合分析的方法对标准物质进行定值。研制的氯霉素标准物质的纯度为99.7%,定值结果不确定度为0.3%(k=2)。
The reference material of chloramphenicol was characterized by melting method, mass spectrometry and infrared spectroscopy. The purity was determined by high performance liquid chromatography （HPLC） ,the separation and detection conditions were studied. The homogeneity and stability of the reference material were described. The results showed that chloramphenicol stability was better in metheyl cyanide than in methyl alcohol. The purity and the expanded combined uncertainty were 99.7% and 0.3% （k = 2） , respectively.
出处
《化学分析计量》
CAS
2008年第1期4-7,共4页
Chemical Analysis And Meterage
关键词
氯霉素
标准物质
纯度
chloramphenicol, reference material, purity
