犬心脏移植围术期血清IL-6、IL-8、TNF-α的变化

The perioperative changes of serum IL-6、IL-8 and TNFα in caine heart transplantion

目的探讨犬心脏移植手术中血清白细胞介质6(interleukin-6,IL-6)、白细胞介质8(interleukin-8,IL-8)、肿瘤坏死因子(αtumor necrosis factor-α,TNF-α)浓度的变化。方法20只家犬随机分组配对进行心脏移植手术,分别在麻醉后、转机时、停机时、手术结束时取动脉血用酶联免疫吸附法(ELISA)检测受体犬血清中的IL-6I、L-8、TNF-α浓度。结果犬血清IL-6I、L-8、TNF-α浓度在麻醉后持续上升,以停机时和手术结束时更为明显(P<0.05)。结论犬心脏移植手术中存在明显的应激反应和缺血再灌注损伤。

Objective To study the changes of serum IL-6, IL-8 and TNF-α in orthotopic heart transplantation in canines. Methods Twenty dogs were assigned to two groups to accept orthotopic heart transplantation respectively, arterial blood samples were obtained at the moment of after anesthesia, the beginning of CPB, the end of CPB, and post-operation. To analyze the serum concentrations of IL-6, IL-8 and TNF-α with ELISA. Results The concentrations of IL-6, IL-8, TNF-α were kept on rising after anesthesia, and at the end of CPB and post-operation their concentratons were striking（ P 〈 0.05 ）. Conclusion Stress reaction and ischemical reperfusion injury were significantly happened in orthotopic heart transplantation of dogs.