高产谷胱甘肽面包酵母菌种的筛选与发酵条件优化

SCREENING OF HIGHER GLUTATHIONE-PRODUCING STRAIN AND FERMENTATION CONDITION OPTIMIZED

通过摇瓶试验筛选出谷胱甘肽含量较高的面包酵母BY-14为试验菌株,对产谷胱甘肽的发酵条件进行了优化,其最适的培养条件为:转速160r/min,发酵时间22h,温度28℃,接种量10%,装液量50mL。在优化的条件下,谷胱甘肽的含量达到16.04mg/g,为初始条件下的1.48倍。

Take this laboratory glutathione content highest strain BY-14 as the experimental strain. The factors that affected the biomass and glutathione content of the fusant BY-14 were tested, the determination optimizes after the culture condition is： rotate speed 160 r/min,cuhure time 22 h, culture temperature 28 ℃, inoculum size 10 %, medium volumes 50 mL/250 mL.Glutathione yield under the optimal fermentation condition showed a 1.48 fold improvement over that of the initial condition.