辣椒制品中苏丹红Ⅰ～Ⅳ号检测方法比较研究

CONTRASTIVE ANALYSIS OF DETERMINATION METHOD OF SUDAN Ⅰ-Ⅳ IN FOOD OF CAPSICUM

实验选用高效液相色谱法(HPLC)和液相色谱-质谱联用法(LC-MS)两种方法对辣椒制品中苏丹红Ⅰ～Ⅳ号进行了分离和检测。HPLC法选用229nm作为苏丹红Ⅰ～Ⅳ号的检测波长,其工作曲线的线性范围较宽,分别为0.625ng～2000ng(R2=1)、2.5ng～2000ng(R2=1)、5ng～2000ng(R2=0.9989)和5ng～2000ng(R2=0.9990),苏丹红Ⅰ～Ⅳ号的检测限分别为1.88μg/kg、2.5μg/kg、6.25μg/kg和10μg/kg,适合含量较高的样品检测。LC-MS法在质核比(M/Z)为249.05、277.10、353.10和381.15的条件下分别对苏丹红Ⅰ～Ⅳ号进行MS检测,其工作曲线线性范围较窄,苏丹红Ⅰ～Ⅲ为0.10ng～5ng(R2≥0.9997),苏丹红Ⅳ号为0.25ng～5ng(R2=0.9998),苏丹红Ⅰ～Ⅳ号的检测限均为1μg/kg,更适合微量样品的检测。两种方法均具有简单、快速的特点。

This study was established two methods based on high performance liquid chromatography （HPLC） and high performance liquid chromatography-mass spectrometry（LC-MS）for determining Sudan Ⅰ -Ⅳ in food. The Sudan Ⅰ -Ⅳ were detected at 229 nm in HPLC. The calibration curve of Sudan Ⅰ -Ⅳ were showed good linearity in the difference range of 0.625 ng-2 000 ng （R^2=1）, 2.5 ng-2 000 ng （R^2=1）, 5 ng-2 000 ng （R^2=0.998 9） and 5 ng-2 000 ng （W=0.9990）.The detection limits of Sudan Ⅰ -Ⅳ were 1.88 μg/kg, 2.5 μg/kg, 6.25 μg/kg and 10 μg/kg, respectively. It is applied for analysis of higher content samples .The Sudan Ⅰ -Ⅳ were detected SIM （ ＋ ） at 249.05,277.10,353.10 and 381.15 （ M/Z ） in LC-MS ,respectively. The calibration curve of Sudan Ⅰ -Ⅲ were showed good linearity in the difference range of 0.10 ng-5 ng （R^2≥0.999 7）, but Sudan IV was 0.25 ng-5 ng （R^2=0.999 8）.The detection limits of Sudan Ⅰ -Ⅳwere at 1 μg/kg, it is applied for analysis of microamount sample.These methods are simple and quickly.