白血病电镜诊断学的建立及应用

Establishment and Application of ElectronMicroscopic Diagnosis on Leukamia

电镜诊断学是指对临床某些疑难病例，应用综合电镜技术，列出标本制备常规流程，确定诊断指标及鉴别诊断指标，在超微结构水平上作出明确诊断的一门新兴应用方法学。我们自７０年代初开始，对白血病进行了系列的电镜观察，建立了适用于白血病诊断的综合电镜技术，确定了可靠的诊断指标。经１０００多例次的电镜诊断和临床疗效验证，其确诊率由原来用光镜分类的６０～７０％（按ＦＡＢ分类）提高到９９％以上。该方法学主要内容包括：白血病超微结构形态分类、酶细胞化学诊断、免疫细胞化学分型诊断、电镜标本制作常规流程。

Electron microscopic diagnosis is a new methodology, it is a new method to apply synthetical electron microscopy to work out routine technological process of samples preparation and determine diagnostic and differential standard to achive a positive assessment on ultrastructural levels for clinical difficult diagnosis diseases. We have observed more than 1000 cases of leukamia using these synthetical electron microscopy from 1970 years. The rate of corrective diagnose have been risen to over 99%. Till then, the rate of the light microscopic diagnose was only 60—70% depended on FAB. This synthetical method include the ultrastructural morphological classification, enzyme cytochemistry diagnosis and immunocytochemistry classification and routine process of electron microscopic samples preparation on leukamia.