姜黄素通过阻断NF-κB减少IL-1β诱导的内皮脂酶表达

Curcumin attenuates IL-1β-induced endothelial lipase expression via blocking NF-κB pathway

目的观察姜黄素对培养的人血管内皮细胞内皮脂酶表达的影响,并探讨其可能的作用机制。方法不同浓度的姜黄素处理HUVEC-12细胞。RT-PCR检测内皮酯酶(endo-thelial lipase,EL)mRNA的表达;Western blot检测核因子-κB抑制因子-α(inhibitor of nuclear factor-κB-α,IκB-α)蛋白的表达;间接免疫荧光检测核因子-κB(nuclear factor-κB,NF-κB)蛋白的活化。结果IL-1β处理HUVEC-12细胞可以明显上调EL mRNA的表达,同时降低胞质蛋白IκB-α的表达水平,激活核转录因子NF-κB,增加胞核蛋白NF-κB的水平。姜黄素预处理HUVEC-12细胞可以抑制IL-1β对EL的上调作用,同时逆转IL-1β诱导的IκB-α蛋白降解和NF-κB活化。结论姜黄素可以通过阻断NF-κB活化减少IL-1β诱导的人血管内皮细胞EL的表达。

Aim To investigate the effect and the possible mechanisms involved in the effect of curcumin on endothelial lipase （EL） expression in human umbilical vein endothelial cells line-12 （HUVEC-12）. Methods HUVEC-12 were pretreated with different concentri-tions of curcumin （0- 100 ted with or without IL-1β. μmol·L^ -1 ） and then treated with or without IL-1β.eNODTHELIAL LIPASE（EL） expression was detected by RT-PCR analysis.The expression of inhibitor of nuclear factor-κB -α（IκB-α） was assayed by Western blot .Nuclear factor-κb（NF-κB） was analyzed by Immunofluorescence. Results IL-1β significantly induced EL expression at mRNA level as compared with control group. The IL-1β induction of EL expression was accompanied by activation of NF-κB and reduction of IκB-α. Cureumin almost completely blocked IL-1β-induced EL expression with a marked reduction of NF-κB and significant induction of IκB-α. Conclusion Curcumin inhibited IL-1β-induced EL expression via blocking NF-κB pathway in HUVEC-12 cells.