多效生长因子对基质金属蛋白酶3,10的负调控作用

Pleiotrophin Negative Regulate the Matrix Metalloproteinase 3 and 10

目的:研究多效生长因子对基质金属蛋白酶(MMP)3、10的负调控作用。方法:用RT-PCR、Northern印迹比较对照与多效生长因子沉默细胞中MMP3、MMP10的表达;在多效生长因子沉默细胞培养液中加入50 ng/mL多效生长因子,检测MMP3、MMP10的表达。结果:多效生长因子缺失细胞中MMP3、MMP10高表达;而在其中添加多效生长因子之后,MMP3、MMP10的表达基本被完全抑制。结论:多效生长因子对MMP3、MMP10有负调控作用。

Objective： To investigate the transcription regulation of matrix metalloproteinase（MMP） 3, 10 by pleiotrophin （PTN）. Methods： The mRNA expression levels of MMP3, MMP10 were compared between control and PTN knocking down MEF cells by RT-PCR and Northern Blot. Then, 50 ng/mL PTN was added in culture medium of the PTN knocking down MEF cells to investigate the effect of pleiotrophin on the transcription of MMP3, MMP10. Results： MMP3, MMP10 expressed higher in the cell that PTN was absence, but they expressed lower after the PTN effeeted. Conclusion： PTN negative regulated the expression of MMP3 and MMP10.