谷氨酸棒杆菌TL1105的L-组氨酸生物合成途径分析

Pathway Analysis for Production of L-Histidine by Corynebacterium glutamicum TL1105

目的:对谷氨酸棒杆菌TL1105由葡萄糖生物合成L-组氨酸的代谢途径进行分析,以确定L-组氨酸合成的最佳途径和最大理论产率。方法:运用METATOOL软件对谷氨酸棒杆菌TL1105合成L-组氨酸进行途径分析。结果:确定了L-组氨酸合成的最佳途径,并确定最大理论产率为1.2;通过比较途径分析所获得的基础反应模型,确定了5-磷酸核糖焦磷酸是L-组氨酸合成途径的关键节点,并且确定了谷氨酸的大量合成是L-组氨酸合成的重要前提;添加谷氨酸,L-组氨酸的产量提高了39.2%。结论:以途径分析为指导,改变外界环境因子,L-组氨酸的产量得到显著的提高。

Objective： Pathway analysis was conducted to determine theoretically optimal mode for the L-histidine production by Corynebacterium glutamicum TL1105. Methods： METATOOL was used to analyze the L-histidine biosynthesis pathway in C.glutamicum TLll05. Results： Theoretically optimal mode and theoretical L-histidine biosynthesis yield of 1.2 were determined. The phosphoribosyl pyrophosphate（PRPP） was found to be the key node of L-histidine biosynthesis pathway by analyzing all the different elementary modes and the flux of L-histidine could be largely increased by changing the environmental factors. The production of L-histidine in fermentation broth increased 39.2% with the addition of L-glutamate than that in the original conditions. Conclusion＂ According to the pathway analysis result, the production of L-histidine can be largely increased by changing the environmental factors.