子痫前期患者胎盘和脐动脉组织Rho相关蛋白激酶Ⅱ的表达及意义

Expression and significance of Rho-associated protein kinase Ⅱ in preeclamptic placenta and umbilical artery

目的探讨子痫前期患者胎盘和脐动脉组织中Rho相关蛋白激酶Ⅱ（RockⅡ）的表达变化及其意义。方法采用RT-PCR、蛋白印迹法分别检测35例轻度子痫前期患者（轻度子痫前期组）、38例重度子痫前期患者（重度子痫前期组）及45例正常晚期妊娠妇女（对照组）胎盘及脐动脉组织中RockⅡmRNA及其蛋白表达水平；采用免疫组化方法对RockⅡ在胎盘组织中的表达进行定位，B超检测脐动脉收缩期最大血流速度（S）与舒张末期血流速度（D）的比值。结果（1）RockⅡ主要表达于胎盘合体滋养细胞的细胞质。（2）轻度子痫前期组、重度子痫前期组及对照组胎盘组织RockⅡmRNA表达水平分别为0．82±0．14、0、93±0．13及0．70±0．12，分别比较，差异也均有统计学意义（P〈0．01）；轻度子痫前期组、重度子痫前期组及对照组胎盘组织RockⅡ蛋白表达水平分别为0．79±0．15、0．92±0．12及0．68±0．11，分别比较，差异也均有统计学意义（P〈0．01）。（3）轻度子痫前期组、重度子痫前期组及对照组脐动脉组织RockⅡmRNA表达水平分别为0．69±0．13、0．55±0．12及0．76±0．10，分别比较，差异也均有统计学意义（P〈0．01）；轻度子痫前期组、重度子痫前期组及对照组脐动脉组织RockⅡ蛋白表达水平分别为0．68±0．10、0、51±0．12及0．75±0．13，分别比较，差异也均有统计学意义（P〈0．01）。（4）脐动脉组织RockⅡmRNA及蛋白表达水平与脐动脉S／D值均无相关性（P〉0．05）。结论RockⅡ在子痫前期患者胎盘组织中的表达水平升高，在脐动脉组织中的表达水平降低，可能参与了子痫前期的病理、生理过程。

Objective To investigate the expression and significance of Rho-associated protein kinase Ⅱ （ Rock Ⅱ ） in preeclamptic placenta and umbilical artery. Methods Semiquantitative RT-PCR and Western blot were used to investigate the expression of Rock Ⅱ mRNA and Rock Ⅱ protein in placenta and umbilical artery from 35 women with moderate preeclampsia （MPE group ）, 38 women with severe preeclampsia（ SPE group） and 45 normal third trimester pregnant women （ control group）, the S/D value of umbilical artery was examined by ultrasound. Results （ 1 ） The expression of Rock Ⅱ mRNA of placenta in MPE group （0. 82 ± 0. 14）and SPE group（0. 93 ± 0. 13） were significantly higher than that in control group （0.70 ± 0. 12,P 〈 0. 01 ）. The expression of Rock Ⅱ protein of placenta in MPE group （0. 79 ± 0. 15 ） and SPE group（0. 92 ± 0. 12） were significantly higher compared with control group（ 0. 68 ± 0. 11 ,P 〈 0. 01 ）. The expression of Rock Ⅱ mRNA and protein of placenta in SPE group were higher compared with MPE group （P 〈 0. 01 ）. （2） The expression of Rock Ⅱ mRNA of umbilical artery in MPE group （0. 69 ± 0. 13 ） and SPE group（0. 55 ± 0. 12 ） were significantly lower than that in control group （0. 76 ± 0. 10, P 〈 0. 01 ）. The expression of Rock Ⅱ protein of umbilical artery in MPE group（ 0. 68 ± 0. 10 ）and SPE group （0. 51 ± 0. 12）were lower compared with control group（0. 75 ± 0. 13 ,P 〈 0. 01 ）. The expression of Rock Ⅱ mRNA and protein of umbilical artery in SPE group were significantly lower compared with MPE group（ P 〈 0. 01 ）. （3） There were no correlations between the expression of Rock Ⅱ mRNA and protein in placenta and umbilical artery and the S/D value and birth weight（ P 〉 0.05 ）. Conclusion The upregulated expression of Rock Ⅱ in placentas and downregulated expression in umbilical artery may be a compensation in preeclampsia.