TFPI-2基因表达变化与声门上喉癌侵袭转移及预后的相关性研究

Correlation of TFPI-2 gene expression with invasion, metastasis, and prognosis of supraglottic carcinoma

目的：探讨TFPI-2基因的表达与声门上喉癌侵袭转移及预后的关系。方法：运用反转录-聚合酶链反应（RT—PCR）及免疫组织化学方法检测TFPI-2在声门上喉癌组织中的表达，分析其与声门上喉癌临床病理特征的关系。结果：TFPI-2在声门上喉癌组织中mRNA和蛋白表达的阳性率明显增加（P〈0．05），TFPI-2 mRNA和蛋白表达随声门上喉癌的浸润深度的增加而降低（P〈0．05），在淋巴结转移组低于非淋巴结转移组（P〈0．05），在临床Ⅲ-Ⅳ期的表达低于临床Ⅰ-Ⅱ期（P〈0．05）。TFPI-2蛋白染色强度随声门上喉癌的侵袭程度增加及淋巴结转移而降低（P〈0．05）。TFPI-2蛋白表达阴性组声门上喉癌患者较阳性组者预后差（P〈0．05）。结论：TFPI-2表达下调可能与声门上喉癌的侵袭转移密切相关，可作为评估声门上喉癌淋巴结转移及预后的指标。

Objective：To investigate the relationship between TFPI-2 gene expression and the invasion, metastasis, and prognosis of supraglottic carcinoma. Methods： Reverse transcriptase polymerase chain reaction and immunohistochemical methods were used to examine the expression of TFPI-2 in supraglottic carcinoma, and the relationship between TFPI-2 expression and the clinicopathological features of supraglottic carcinoma were analyzed. Results ： Positive ratio of TFPI-2 expression was significantly increased at protein and mRNA levels in supraglottic carcinoma tissues （P 〈 0.05 ）. TFPI-2 mRNA and protein expression were decreased with the increase in the invasive depth of supraglottic carcinoma （ P 〈 0.05 ）. TFPI-2 mRNA and protein expression were significantly lower in supraglottic carcinoma tissues with nodal metastasis and at Ⅲ-Ⅳ clinical stage than those without nodal metastasis and at Ⅰ-Ⅱ clinical stage （ P 〈 0.05）. The staining intensity of TFPI-2 protein was also decreased with increase in invasive depth and nodal metastasis （ P 〈 0.05）. The supraglottic carcinoma patients with negative TFPI-2 protein expression had poor prognosis than those with positive TFPI-2 expression. Conclusion： The downregulation of TFPI-2 has a close relationship with invasion and metastasis of supraglottic carcinoma, and it may serve as a helpful marker in evaluating the metastasis and prognosis of supraglottic carcinoma.