CDC 25A和CDC 25B基因在宫颈癌中的表达和临床意义

Expressions of CDC 25A and CDC 25B genes in cervical cancer and their clinical significance

目的：检测及讨论CDC 25A和CDC 25B基因在宫颈癌中的表达水平及其临床意义。分析CDC 25A和CDC 25B基因剪切变异分子CDC 25A1—2、CDC 2581—4在宫颈组织中的表达分布规律。方法：采用RT—PCR和Western印迹法检测61例宫颈癌组织、18例宫颈良性病变组织中CDC 25的表达情况，并分析CDC 25的表达水平与宫颈癌临床病理参数之间的关系。结果：CDC 25A和CDC 25B基因在宫颈癌组织和宫颈良性病变组织中的表达差异有统计学意义（P〈0．05）；CDC 25的各剪切变异分子表达在宫颈癌患者不同年龄、癌组织病理类型、临床分期及癌细胞分化程度间差异有统计学意义（P〈0．05），而与宫颈癌的淋巴结转移无关（P〉0．05）。结论：宫颈癌组织中存在CDC 25A和B的高表达，CDC 25分子及其剪切变异体的表达异常可能是宫颈癌发生、发展的重要细胞内调控机制之一。

Objective：To detect the expression levels of CDC 25A and CDC 25B and their clinical significance and analyze the distribution pattern of the expressions of their splicing variants CDC 25A1-2 and CDC 25B1-4 in cervical tissues. Methods：The mRNA and protein expressions of CDC 25s were detected in 61 cases of cervical cancer tissues and 18 cases of benign cervical disease by RT- PCR and Western blotting, respectively. The relationship between the expression of CDC 25s and the clinicopathological features of cervical cancer was analyzed by the statistical method. Results：The difference in the expression levels of CDC 25A and CDC 25B between cervical cancer tissues and benign cervical diseases was significant （ P 〈0.05 ）. The expression level of CDC 25s splicing variants had significant correlation with age of cervical cancer patients, histological classification, clinicopathological staging, and differentiation of cervical cancer cells （P 〈 0.05 ）, but had no correlation with lymph node metastasis in cervical cancer （ P 〉 0.05 ）. Conclusion： The CDC 25A and CDC 25 B are highly expressed in cervical cancer tissues compared with normal cervical tissues. The abnormal expressions of CDC 25s and the splicing variants may be one of the important intracellular regulating mechanisms for the tumorigenesis and development of cervical cancer.