P型铜转运ATP酶在卵巢癌中的表达及其与化疗耐药的关系

The expression of copper-transporting P-type adenosine triphosphatase in ovarian cancer and its correlation with chemotherapy resistance

目的:探讨P型铜转运ATP酶(copper-transporting P-type adenosine triphosphatase,ATP 7B)在卵巢癌组织中的表达及其与临床病理参数、化疗耐药的关系。方法:用半定量RT-PCR技术检测ATP 7B在36例卵巢癌组织,10例良性卵巢肿瘤组织,10例正常卵巢组织中的表达水平。结果:ATP 7B在卵巢癌组织中的阳性表达率为38.9%,显著高于在良性卵巢肿瘤(0%)及正常卵巢组织中(0%)的表达(P<0.01)。ATP 7B的表达与肿瘤分化程度有关,ATP 7B在低分化组织中的表达明显高于高、中分化组(P<0.05)。ATP7B在术前化疗组中的表达高于术前未化疗组,差异有统计学意义(P<0.05)。ATP7B表达阳性的卵巢癌患者对化疗的反应率(28.6%)明显低于表达为阴性的患者(72.7%)(P<0.05)。结论:ATP 7B可能在卵巢癌对铂类抗肿瘤药的耐药过程中发挥了重要作用。

Objective：To investigate the expression of copper-transporting P-type adenosine triphosphatase （ATP 7B） in the ovarian cancer and evaluate its relationship with chemo-resistance and the clinicopathologic parameters of ovarian carcinoma. Methods： Reverse transcription polymerase chain reaction（RT-PCR） was used to detect the expression of ATP 7B in 36 cases of ovarian carcinoma, 10 cases of benign ovarian tumor, and 10 cases of normal ovarian tissue. Results：The positive expression rate of ATPTB in ovarian carcinoma was 38.9% significantly higher than that in benign ovarian tumor （0%） and normal ovarian tissue （0%） （P 〈 0.01 ）. There was a significant correlation between ATP 7 B expression and differentiation degree. ATP 7 B expression was significantly higher in poorly differentiated carcinoma than that in well and moderate differentiated carcinoma （ P 〈 0. 05 ）. The expression of ATP 7 B was more positive in patients who received chemotherapy before surgery than those who did not receive chemotherapy. The difference was significant （P 〈 0.05）. The response rate of ATP 7B-positive patients to chemotherapy was significantly lower than the ATP 7B-negative patients （28.6% vs 72.7%, P 〈 0.05）. Conclusion：These data suggested that ATP 7B plays an important role in the multidrug resistance of the ovarian carcinoma to Pt-based chemotherapy.