MRI评价bFGF、银杏叶提取物联合应用治疗猫急性脑梗死的实验研究

MRI evaluation on the treatment of acute cerebral infarction of cat with the combination of bFGF and GbE

目的:利用MR影像学方法对碱性成纤维细胞生长因子(bFGF)、银杏叶提取物(GbE)联合治疗缺血性脑损伤的作用进行评价。方法:40只健康家猫随机平均分为4组。A组(生理盐水对照组),B组(bFGF处理组),C组(GbE处理组),D组(bFGF+GbE处理组)。采用左侧眼眶入路制作大脑中动脉缺血再灌注模型。术后动态进行T1WI、T2WI、FLAIR、DWI序列扫描,并进行神经功能缺损评分。结果:联合治疗组神经功能缺损程度轻,磁共振T1WI、T2WI、FLAIR所见病变程度轻,T2WI显示最终梗死体积小,T2WI-ADC显示梗死周围缺血带转化为梗死的体积小。结论:bFGF、GbE联合治疗猫急性脑梗死优于单一用药组,两者具有协同作用;MRI能客观评价药物疗效,尤其T2WI-ADC异常范围差值是评价药物疗效的重要指标。

Objective： To evaluate the therapeutic effect of bFGF and GbE on acute cerebral infarction with MR imaging. Methods： Forty healthy adult cats were equally and randomly derided into 4 groups. All animals were operated to establish the feline models of cerebral focal ischemia-reperfusion by left transorbital approach. They were injected with sodium chloride intravenously for group A, bFGF for group B, GbE for group C and bFGF＋GbE for group D. MR scans performed after operation, included T1WI, T2WI, FLAIR, DWI. Philips＇s criteria score were evaluated on the cats. Result： The neurological defects score in group D is remarkably lower than that in group B and C. The lesion on T1WI, T2WI, FLAIR, DWI showed more milder. The area of infarct formation were smaller than that in group B and C through the comparison between the ADC images and T2WI images （T2WI-ADC）. Conclusion： The treatment results in group D was better than that in group B and C. Both bFGF and GbE can enhance each other＇s therapeutic effects. MR imaging could show the therapeutic effect of bFGF and GbE, especially the T2WI-ADC was an important item to evaluate the therapeutic effects.