广州市一起肾综合症出血热实验室感染事件的调查与分析

An Investigation and Analysis of lab infection affair of HFRS in Guangzhou,China.

目的通过一起典型案例的调查与分析,探讨肾综合症出血热实验室感染的原因及流行病学特征,为类似感染事件提供相应的调查和处理模式。方法采用现场流行病学调查方法对广州市2007年一起流行性出血热实验室感染事件进行调查;人血标本采用间接免疫荧光方法检测,鼠肺组织抗原采用直接免疫荧光检测,鼠血标本采用反相间接血凝法(RPHI)检测。结果患者临床表现和实验室检测结果符合肾综合症出血热诊断标准;流行病学调查证据支持实验室感染可能;开放饲养区采集的43份鼠肺标本荧光PCR检测,其中4份标本阳性;8份野鼠血有2份阳性;动物供应单位鼠血清标本抗体检测,40份均为阴性;实验室相关人员标本60份中1份标本IgG抗体阳性。结论本次事件为感染肾综合症出血热的野鼠将病毒传播给开放饲养区的实验动物,而患者与该区实验动物接触而发病。

Objective To explore the cause of lab infection and epidemic character though investigating and analyzing a typical case and to provide the investigation and treat model for the similar affairs. Methods A lab infection affair of HFRS in Guangzhou was investigated in 2007 by field epidemic method. The human serum sample was detected by indirect immunofluorescence method, the mice lung sample by direct immunofluoresence Methods and the mice serum sample by RPHI method. Results The clinic manifestations and lab results accord with the diagnostic criteria of HFRS. The evidence of epidemic investigation also supported the possibility of lab infection. 4 of 43 mice lung sample in open feeding area were positive and 2 of 8 field mice serum sample were positive also. While the 40 mice sample from the animal providing institution were all negative. And one serum sample of IgG antibody among 60 lab staff was positive. Conclusion This affair was arisen by the field mice transmitting the virus of HFRS to the lab animal in the open area and the sufferer contactted those infection animal closely and then been attacked.