甘蓝型油菜主要脂肪酸组成的QTL定位

QTL Identification for Fatty Acid Content in Rapeseed(Brassica napus L.)

应用RAPD、SSR和SRAP技术,对甘蓝型油菜低芥酸品系APL01与高芥酸品系M083杂交组合的BC1F1群体进行检测,获得251个分子标记,构建了19个连锁群组成的分子标记遗传图谱;应用WinQTLCart2.0对油菜主要脂肪酸组成进行QTL扫描,获得与棕榈酸含量相关的QTL5个,分别位于N3、N8、N10和N13连锁群,其中效应值较大的主效QTLqPA8-1和qPA13分别可解释棕榈酸含量表型变异的11.31%和14.47%。获得与硬脂酸含量相关的QTL3个,分别位于N1、N8和N16连锁群,其中效应值较大的主效QTLqST16可解释硬脂酸含量表型变异的12.22%。获得与油酸含量相关的QTL2个,位于N8和N13连锁群,均为主效QTL,其中qOL8位于N8连锁群的m11e37b^A0226Ba267区间,可解释油酸含量表型变异的11.73%,qOL13位于N13连锁群的m18e46~m20e25a区间,可解释表型变异的27.14%。获得与亚油酸含量相关的QTL3个,其中主效QTLqLI8-1位于N8连锁群,可解释亚油酸含量表型变异的13.25%。获得与亚麻酸含量相关的QTL3个,效应值均较小,属微效QTL。获得与廿碳烯酸含量相关的QTL4个,分别位于N8、N13和N15连锁群,其中主效QTLqEI8-1、qEI8-2和qEI13分别可解释廿碳烯酸含量表型变异的12.20%、10.22%和11.14%。获得与芥酸含量相关的QTL2个,位于N8和N13连锁群,均为主效QTL,其中qER8位于N8连锁群的m11e37b^A0226Ba267区间,可解释芥酸含量表型变异的16.74%;qER13位于N13连锁群的A0301Bb398~m18e46区间,可解释芥酸含量表型变异的31.32%。在N8连锁群的分子标记m11e27b附近及N13连锁群的分子标记m18e46附近存在多个主要脂肪酸的主效QTL,这些标记可用于油菜脂肪酸改良的分子标记辅助选择。

Rapeseed is one of the important oil crops in China, and the change of the composition in fatty acid content can affect the value of oil for edible and industrial use. QTLs linked to main fatty acid composition can be used for marker-assisted selection in fatty acid composition improvement in rapeseed. A segregation population BC1F1, derivered from a cross between a low erucic acid content line APL01 and a high erucic acid content variety M083 in Brassica napus L., was used to construct a genetic map and identify QTLs linked to main fatty acid composition in rapeseed. The genetic map containing 19 linkage groups was constructed with 251 markers of RAPD, SSR, and SRAP in rapeseed. Via WinQTLCart scanning, five QTLs linked to palmitic acid （C16：0） content were identified, which were located on linkage groups N3, N8, N10, and N13.The main-effect QTLs qPAS-1 and qPA13 linked to palmatic acid content in rapeseed explained 11.31% and 14.47% of the palmitic acid content variation in segregation population BC1F1, respectively. Three QTLs linked to stearic acid （C18：0） content were identified, which were located on the linkage groups N1, NS, and N16. The main-effect QTL qST16 accounted for 12.22% of phenotypic variation. Two QTLs, qOL8 and qOL13, both the main-effect QTLs linked to oleic acid （C18：1） content, were located in the region of mlle37b-A0226Ba267 on linkage group N8 and m18e46-m20e25a on N13, and accounted for 11.73% and 27.14% of phenotypic variation, respectively. Three QTLs linked to linoleic acid （C18：2） content were identified, and the main-effect QTL qL18-1 accounted for 13.25% of phenotypic variation. Three QTLs, qLN1, qLN8, and qLNll, linked to linolenic acid （C18：3） content were identified, but they had a rather small effects on C18：3, and could be regarded as minor-QTLs. Four QTLs linked to eicosenoic acid （C20：1） content, and three of them, qE18-1, qE18-2, and qE113, were main-effect QTLs, accounting for 12.20%, 10.22%, and 11.14% of phenotypic variation, respectively. Two QTLs qER8 and qER13, both the main-effect QTLs linked to erucic acid （C22：1） content, were located in the region of m11e37b-A0226Ba267 on linkage group N8 and A0301Bb398-m18e46 on N13, accounting for 16.74% and 31.32% of phenotypic variation, respectively. The main-effect QTLs linked to fatty acid content, which were located in the region near marker mlle27b on linkage group N8 and m18e46 on linkage group N13, could be used in marker-assisted selection in fatty acid improvement in rapeseed.