摘要
目的:研究小鼠子宫内标记滞留细胞的存在及其分布特点。方法选择出生3天的雌性昆明白小乳鼠,实验组乳鼠皮下注射B rdU 50μg/g,对照组皮下注射生理盐水100μl,每天2次,共注射3d。于最后一次注射后2h、1w、2w、4w和8w处死并取子宫固定,石蜡包埋用于免疫组化检测,每个时间段5只乳鼠。结果实验组标记后2h,小鼠腺体和基质中标记滞留细胞(LRCs)表达最高,分别为45.1%和57.4%,随着时间的增加,LRCs逐渐降低,到第8w在腺体中仅有极少量细胞表达,而基质细胞约有1.5%的LRCs,主要位于血管周围和内膜基层连接处。不同时相腺体和基质细胞中LRCs差异具有显著性(P<0.05)。结论昆明白小鼠子宫内LRCs主要位于血管周围和内膜基层连接处等,这些LRCs可能为子宫内膜干细胞。
Objective： To study the existence of endometrial stem cell and its distribution in mouse uterus. Methods ： Selected female Kunming white mice after boru three days, subcutaneous injected BrdU 50ug/g in experiment group and 0. 9% NS in control group twice daily and totally injected 3 d. Put mice death after injected 2h, tw, 2w, 4w 和 8w, fixed the uterus for immunohistochemistry and pathology, 5. mice for each phase. Results： The expression of LRCs in stroma and gland cells after 2h were 45.1% and 57.4% respectively and which were gradually degraded, Only few LRCs in the gland bottom in 8w and 1.5% LRCs in stroma cells mainly located around the blood vessel and endometrial - myometrial junction. LRCs in different phases of stroma and gland cells were significance （ P 〈 0. 05 ）. Conclusion： There were endometrial stem cells in the uterus of Kunming white mice and mainly located around the blood vessel and endometrial - myometrial junction.
出处
《中国优生与遗传杂志》
2008年第2期53-55,共3页
Chinese Journal of Birth Health & Heredity
基金
国家自然基金(c30600188)
