子宫内膜异位症患者腹腔液中IGF-Ⅰ和IGF-Ⅱ水平的检测与意义

The examination and significance of IGF-Ⅰand IGF-Ⅱin the peritoneal fluid of patients with endometriosis

目的探讨腹腔液中IGF-Ⅰ和IGF-Ⅱ的表达水平在子宫内膜异位症发病中的作用,及其与内异症性不孕的关系。方法采用酶联免疫吸附法(ELISA)检测31例内异症患者(不孕者13例,非不孕者18例)和19例非内异症患者腹腔液中IGF-Ⅰ和IGF-Ⅱ的表达水平。结果内异症组腹腔液中IGF-Ⅰ表达水平(143.59±53.80ng/m l)显著高于对照组(94.44±45.81ng/m l)(P<0.01),并且内异症组中不孕患者IGF-Ⅰ表达水平(174.98±38.47ng/m l)显著高于非不孕者(124.40±53.57ng/m l)(P<0.05),内异症组早晚期IGF-Ⅰ表达水平无显著差异;腹腔液中IGF-Ⅱ表达水平在两组中均无显著差异(P>0.05)。结论IGF-Ⅰ在内异症的发生发展中可能起了一定作用,并且可能是内异症性不孕的一个重要病因。

Objective： To investigate the roles of IGF-Ⅰ and IGF- Ⅱ in peritoneal fluid on pathogenesis of endmetfiosis （EMS） and relationship with infertility of EMS. Methods： IGF-Ⅰ and IGF-Ⅱlevels in peritoneal fluid of 31 eases with EMS were detected by enzyme linked immunoabsorbent assay （ELISA） and compared with the counterpart of 19 cases without EMS （ control groups）. Results： The IGF-Ⅰ levels in peritoneal fluid were significantly higher in patients with EMS than those in control groups （P〈0.01 ）. And in EMS groups, the IGF -Ⅰ levels in peritoneal fluid of infertile patients were significantly higher than those of fertile ones （ P 〈 0. 05 ）. There were no significant differ levels of IGF -Ⅰ between early - stage EMS and late - stage EMS. And IGF -Ⅱlevels in peritoneal fluid were not significant differ between EMS groups and control groups （ P〉 0.05 ）. Conclusion： It was concluded that the increase levels of IGF -Ⅰ in peritoneal fluid maybe involved in pathogenesis of EMS, and was a important cause of infertile of EMS.