鼠巨细胞病毒感染对雄性小鼠生殖性激素水平的影响

Effect of MCMV infection on reproductive sex Hormone in male mice

目的研究鼠巨细胞病毒（MCMV）感染后雄性小鼠生殖性激素水平的变化，探讨MCMV感染影响雄性生殖功能的可能机制。方法选择无McMV活动感染的雄性小鼠，睾丸接种MCMV或不合MCMV的细胞培养液，设立实验组与对照组，采用放射免疫法检测接种后不同时段（小鼠一个生精周期内）的小鼠血清及睾丸组织内性激素含量，并进行比较。结果睾丸匀浆组织T浓度在MCMV感染第4～9d显著降低（P〈0．05），第14～21d有一短暂恢复过程，第38d又较对照组下降（P〈0.05）。血清LH浓度在MCMV感染的第4、9、14d显著升高（P〈0．05），其它时间点比较差异无统计学意义（P〉0．05）。而感染不同时期的血清T和FSH比较差异均无统计学意义（P〉0．05）。结论MCMV急性感染可引起雄鼠生殖内分泌激素水平的改变：通过影响其下丘脑-垂体-性腺轴的调节导致雄鼠生殖功能障碍。

Objective： To study changes of the sex hormone levels after MCMV infection on male mice, to reveal the possible mechanisms of MCMV - induced reproductive handicap. Methods： Following establishment of murine testicular MCMV infection model, experimental and control groups were set. In different periods of MCMV infection （within one seminiferous cycle）, the sex hormone levels in murine sera and testicular tissue was detected by using radioimmunoassay to study the regulatory effects of hypothalamus -pituitary -testis axis on the reproductive function in different periods after MCMV infection as viewed from reproductive biology. Re- suits： In the testicular homogenate, testosterone （T） concentration in the experimental group was obviously decreased on D4 to D9 PI （P〈0.05）, transiently restored on D14 to D21 PI, and on D38 PI, it was decreased as compared with that in the control group （ P〈0.05）. Serum lnteinizing hormone （LH） in the experimental group was significantly increased on D4, D9 and D14 PI （ P〈0.05 ）, but there was no significant difference on the other time points between the experimental groups and control group. Serum T and follicle stimulating hormone （FSH） showed no significant difference among the experimental groups. Conclusion： MCMV acute infection could result in the changes of the sex hormone levels in male mice by influencing the regulatory of hypothalamus - pituitary - testis axis to affect the reproductive function of the mice.