优化亚硝基胍(NTG)诱变条件选育低产蛋白酶A菌株的研究

Study on the Optimization of NTG Mutagenesis Conditions and Breeding Brewing Yeast With Lower-level Porteinase A

通过优化亚硝基胍(NTG)对啤酒酵母的化学诱变条件,得出NTG的最佳实验条件,选育低产蛋白酶A的菌株,比较了两种初筛方法--羧肽酶Y平板实验和酸变性血红蛋白实验的筛选效果,筛选出6株蛋白酶A活力较低的菌株,并进行多次重复性实验检验其稳定性。

This work mainly focused on the most optimized experimental conditions of NTG at first, then the comparison of two screening methods carboxypeptidase Y plate and acid-denatured hemoglobin plate were taken into accounted. Six brewing yeasts with lower-level porteinase A were bred, and the stability of their porteinase A level were evaluated.